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Background1

With approximately 20 million dengue cases a year and 100 countries affected, the World Health Organization considers dengue the most important mosquito-borne viral disease. A vaccine is not currently available and mosquito control is a critical element of dengue disease prevention. There are reports that conventional ground and aerial application of insecticides are not providing adequate control of the mosquitoes that transmit dengue. Dengue is primarily transmitted by the Aedes species of mosquito. The Aedes mosquito is a container breeder and, therefore, the female Aedesmosquito will only lay eggs in a container holding water.

Chemical mosquito control has relied on area treatment of the mosquitoes' habitat. For example, large outdoor areas or the space within a home are treated with an insecticidal fog or spray in an attempt to deposit a lethal dose of insecticide on individual mosquitoes. The water used as mosquito breeding habitats has also been treated with insecticide which is designed to kill the developing larva. Adult resting places have been treated with an insecticide deposit designed to kill adult mosquitoes as they stand on the resting places.

The oviposition trap has been used as a surveillance tool for Aedes mosquitoes.  The ovitrap consists of a pint-sized water container, filled with water to within 1 inch of the top, with a wooden or velour paper paddle projecting above the water surface. Aedes females are attracted to the ovitrap and deposit eggs on the paddle.  The number of eggs deposited provides an indication of the Aedes mosquito population.

US Military research scientists Michael Perich and Brian Zeichner believed that they could use the "female mosquito's irrepressible urge to oviposit" to develop a trap with advantages over conventional methods of controlling the population of container-breeding mosquitoes. By killing adult female container-breeding mosquitoes, the population of biting mosquitoes is substantially reduced, thus reducing both the potential for disease transmission and the breeding stock for the next generation. This approach is superior to simply trapping and removing eggs laid by female container breeding mosquitoes, which may then go on to lay additional eggs in a natural site which allows complete development of the larvae to adults.

map dengue

Dengue Outbreaks in 2005 - CDC Dengue Factsheet


Since a portion of the insecticide can leach into the water, any mosquito larvae which hatch may not survive because the water can be toxic to mosquito larvae.  Furthermore, any mosquito adults drinking from the insecticide-contaminated water may also perish.  In this way, mosquitos which may bypass the insecticide treated egg laying structure can be killed.  In caged laboratory tests, up to 100 percent adult mosquito mortality has occurred.

The Trap-N-Kill® (TNK) containers can easily be made child safe because a very small quantity of insecticide is required.  For example, a 20 pound child can consume ten egg laying structures containing the insecticide and drink the insecticide contaminated water of ten 1 pint containers per day without any effects when using bifenthrin as the insecticide.

The TNK containers can be made environmentally sound; because the amount of insecticide required is low, the mortality rate of mammals is very low and the threat of contamination to ground water or soil is also very low.

The TNK container can be made simple such that community-based control programs can be used to maintain them. In some cases, the only action required will be to periodically replace the insecticide treated egg laying structure, and to add water when evaporation is significant.  In this manner, the manufacturing and maintenance cost of very large numbers of the TNK containers can be made very low because the raw material cost of the insecticide and container is very low.

SpringStar Inc. is the exclusive, world-wide licensee of the intellectual property and patents developed by the Perich-Zeichner Team.


lethal ovitrap

1U.S. Pat. 6,389,740


SpringStar Inc. - PO Box 2622 - Woodinville, WA 98072
(425)-487-6011 or toll-free in the US or Canada (800) 769-1043

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